| Item type |
学内発行雑誌 / Departmental Bulletin Paper(1) |
| 公開日 |
2018-06-21 |
| タイトル |
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タイトル |
Cross-talk between TLR3 and TNF-α or IFN-γ Signaling in Induction of CXCL8/IL-8 and CXCL10/IP-10 Expression in Airway Epithelial Cells |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
departmental bulletin paper |
| 著者 |
HOMMA, Tetsuya
MATSUKURA, Satoshi
HIROSE, Takashi
OHNISHI, Tsukasa
KIMURA, Teruaki
KUROKAWA, Masatsugu
IEKI, Koushi
ODAKA, Miho
SUZUKI, Shintaro
WATANABE, Shin
SATO, Masayuki
KAWAGUCHI, Mio
ADACHI, Mitsuru
|
| 書誌情報 |
The Showa University journal of medical sciences
巻 22,
号 2,
p. 95-103,
発行日 2010-06
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
CXCL8/IL-8 is a chemoattractant for neutrophils and mast cells, and regulates inflammatory cell recruitment in allergy, infection, and other neutrophil-related diseases. Interferon (IFN) -γ-inducible protein 10 (CXCL10/IP-10) is a chemokine that attracts mononuclear cells, Th1 cells, and natural killer cells. We investigated the levels of CXCL8/IL-8 and CXCL10/IP-10 expression by airway epithelial cells after exposure to the inflammatory cytokines tumor necrosis factor (TNF) -α and IFN-γ, and to poly I:C, a synthetic analog of double-stranded RNA that is a ligand of Toll-like receptor 3 (TLR3). Poly I:C, TNF-α, IFN-γ, and combinations of poly I:C with TNF-α or IFN-γ were used to stimulate the airway epithelial cell line BEAS-2B. Following stimulation, we determined CXCL8/IL-8 and CXCL10/IP-10 mRNA levels by real-time PCR and protein levels by ELISA. Poly I:C treatment upregulated mRNA and protein expression for both CXCL8/IL-8 and CXCL10/IP-10. The addition of TNF-α, but not IFN-γ, to poly I:C further increased the expression of CXCL8/IL-8 mRNA and protein. The addition of either TNF-α or IFN-γ to the poly I:C treatment further increased CXCL10/IP-10 mRNA and protein expression. Cross-talk between TLR3 signaling and inflammatory cytokines regulates the expression of CXCL8/IL-8 and CXCL10/IP-10 in airway epithelial cells. From our results, TNF-α and IFN-γ produce different effects on TLR3 signaling. |
| DOI |
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関連識別子 |
10.15369/sujms.22.95 |
| 出版者 |
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出版者 |
Showa Medical Association and Showa University |
| ISSN |
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収録物識別子 |
0915-6380 |
| 著者版フラグ |
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出版タイプ |
VoR |
S22_95.pdf (394.2 kB)
