{"created":"2023-05-15T11:22:04.915171+00:00","id":736,"links":{},"metadata":{"_buckets":{"deposit":"33facc06-e513-4651-8513-8dfaec280067"},"_deposit":{"created_by":1,"id":"736","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"736"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000736","sets":["1:51:94:96"]},"author_link":["4279","4274","4278","4282","4280","4285","4277","4275","4286","4283","4281","4276","4284"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"103","bibliographicPageStart":"95","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CXCL8/IL-8 is a chemoattractant for neutrophils and mast cells, and regulates inflammatory cell recruitment in allergy, infection, and other neutrophil-related diseases. Interferon (IFN) -γ-inducible protein 10 (CXCL10/IP-10) is a chemokine that attracts mononuclear cells, Th1 cells, and natural killer cells. We investigated the levels of CXCL8/IL-8 and CXCL10/IP-10 expression by airway epithelial cells after exposure to the inflammatory cytokines tumor necrosis factor (TNF) -α and IFN-γ, and to poly I:C, a synthetic analog of double-stranded RNA that is a ligand of Toll-like receptor 3 (TLR3). Poly I:C, TNF-α, IFN-γ, and combinations of poly I:C with TNF-α or IFN-γ were used to stimulate the airway epithelial cell line BEAS-2B. Following stimulation, we determined CXCL8/IL-8 and CXCL10/IP-10 mRNA levels by real-time PCR and protein levels by ELISA. Poly I:C treatment upregulated mRNA and protein expression for both CXCL8/IL-8 and CXCL10/IP-10. The addition of TNF-α, but not IFN-γ, to poly I:C further increased the expression of CXCL8/IL-8 mRNA and protein. The addition of either TNF-α or IFN-γ to the poly I:C treatment further increased CXCL10/IP-10 mRNA and protein expression. Cross-talk between TLR3 signaling and inflammatory cytokines regulates the expression of CXCL8/IL-8 and CXCL10/IP-10 in airway epithelial cells. From our results, TNF-α and IFN-γ produce different effects on TLR3 signaling.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa Medical Association and Showa University"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.22.95"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HOMMA, Tetsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUKURA, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIROSE, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHNISHI, Tsukasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIMURA, Teruaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUROKAWA, Masatsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IEKI, Koushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ODAKA, Miho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WATANABE, Shin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SATO, Masayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAWAGUCHI, Mio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ADACHI, Mitsuru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S22_95.pdf","filesize":[{"value":"394.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S22_95.pdf","url":"https://showa.repo.nii.ac.jp/record/736/files/S22_95.pdf"},"version_id":"adb7a5ec-83b6-4df3-9869-868189bbfe70"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Cross-talk between TLR3 and TNF-α or IFN-γ Signaling in Induction of CXCL8/IL-8 and CXCL10/IP-10 Expression in Airway Epithelial Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cross-talk between TLR3 and TNF-α or IFN-γ Signaling in Induction of CXCL8/IL-8 and CXCL10/IP-10 Expression in Airway Epithelial Cells"}]},"item_type_id":"10002","owner":"1","path":["96"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-21"},"publish_date":"2018-06-21","publish_status":"0","recid":"736","relation_version_is_last":true,"title":["Cross-talk between TLR3 and TNF-α or IFN-γ Signaling in Induction of CXCL8/IL-8 and CXCL10/IP-10 Expression in Airway Epithelial Cells"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:36:55.255172+00:00"}