| Item type |
学内発行雑誌 / Departmental Bulletin Paper(1) |
| 公開日 |
2016-12-17 |
| タイトル |
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|
タイトル |
Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ |
departmental bulletin paper |
| 著者 |
AOKI, Kayo
NAKANISHI-UEDA, Takako
TSUJI, Mayumi
OKUNO, Tsutomu
SAITO, Yusuke
YASUHARA, Hajime
|
| 書誌情報 |
The Showa University journal of medical sciences
巻 23,
号 1,
p. 1-10,
発行日 2011-03
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
This study was aimed to determine the ultraviolet (UV: 235-310nm) action spectrum for killing normal human epidermal keratinocytes (NHEK) and to investigate the preventive effect of EPC-K1, a phosphate diester of vitamin C and vitamin E on UV radiation-induced NHEK injury. NHEK were cultured in EpiLife medium supplemented with Human Keratinocyte Growth Supplement Kit. NHEK viability was determined by crystal violet (CV) staining 48 h after the UV irradiation. The mRNA expressions of the C/EBP homologous protein (Chop) transcription factor and endoplasmic reticulum-resident molecular chaperone, Bip, were determined by RT-PCR analyses. UV was especially effective in killing NHEK when applied in the wavelength region of 250-280nm. The minimum exposure dose required to kill 50% of cells (LD50) was 1.64mJ/cm2 at 269nm. At 235 and 310nm, the LD50 for NHEK was 6.62 and 293mJ/cm2, respectively. Irradiation of 660-mJ/cm2 at 310nm significantly decreased the cell viability to 30% of control (without irradiation). The addition of 0.1mM EPC-K1 after irradiation returned the cell viability to 118%. Six hours after the 660-mJ/cm2 irradiation at 310nm, Chop and Bip mRNA levels in NHEK were increased to 487% and 283%, respectively, and were not significantly affected by EPC-K1. Chop and Bip are responsive to ER stress. These results suggested that EPC-K1 exerts a protective effect against UV-induced NHEK injury, and further studies should investigate the molecular mechanism underlying this effect. |
| DOI |
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関連識別子 |
10.15369/sujms.23.1 |
| 出版者 |
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出版者 |
Showa Medical Association and Showa University |
| ISSN |
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収録物識別子 |
0915-6380 |
| 出版タイプ |
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出版タイプ |
VoR |
S23_1.pdf (167.3 kB)
