{"created":"2023-05-15T11:22:03.685192+00:00","id":717,"links":{},"metadata":{"_buckets":{"deposit":"5b6bac43-449c-439d-a7b3-3148e136d129"},"_deposit":{"created_by":1,"id":"717","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"717"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000717","sets":["1:51:89:90"]},"author_link":["4130","4134","4135","4132","4133","4131"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study was aimed to determine the ultraviolet (UV: 235-310nm) action spectrum for killing normal human epidermal keratinocytes (NHEK) and to investigate the preventive effect of EPC-K1, a phosphate diester of vitamin C and vitamin E on UV radiation-induced NHEK injury. NHEK were cultured in EpiLife  medium supplemented with Human Keratinocyte Growth Supplement Kit. NHEK viability was determined by crystal violet (CV) staining 48 h after the UV irradiation. The mRNA expressions of the C/EBP homologous protein (Chop) transcription factor and endoplasmic reticulum-resident molecular chaperone, Bip, were determined by RT-PCR analyses. UV was especially effective in killing NHEK when applied in the wavelength region of 250-280nm. The minimum exposure dose required to kill 50% of cells (LD50) was 1.64mJ/cm2 at 269nm. At 235 and 310nm, the LD50 for NHEK was 6.62 and 293mJ/cm2, respectively. Irradiation of 660-mJ/cm2 at 310nm significantly decreased the cell viability to 30% of control (without irradiation). The addition of 0.1mM EPC-K1 after irradiation returned the cell viability to 118%. Six hours after the 660-mJ/cm2 irradiation at 310nm, Chop and Bip mRNA levels in NHEK were increased to 487% and 283%, respectively, and were not significantly affected by EPC-K1. Chop and Bip are responsive to ER stress. These results suggested that EPC-K1 exerts a protective effect against UV-induced NHEK injury, and further studies should investigate the molecular mechanism underlying this effect.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa Medical Association and Showa University"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.23.1"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"AOKI, Kayo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKANISHI-UEDA, Takako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUJI, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKUNO, Tsutomu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAITO, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YASUHARA, Hajime"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S23_1.pdf","filesize":[{"value":"167.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S23_1.pdf","url":"https://showa.repo.nii.ac.jp/record/717/files/S23_1.pdf"},"version_id":"916702f2-be69-41e8-9c03-9fbca09aff22"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes"}]},"item_type_id":"10002","owner":"1","path":["90"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-12-17"},"publish_date":"2016-12-17","publish_status":"0","recid":"717","relation_version_is_last":true,"title":["Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:37:30.489542+00:00"}