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Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta Synergistically Upregulate Endothelin-1 Expression in Human Bronchial Epithelial Cells BEAS-2B
https://showa.repo.nii.ac.jp/records/547
https://showa.repo.nii.ac.jp/records/547e3df7d40-91a4-4204-9eed-f3c2f487c1bd
名前 / ファイル | ライセンス | アクション |
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S28_101.pdf (443.3 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-02-14 | |||||
タイトル | ||||||
タイトル | Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta Synergistically Upregulate Endothelin-1 Expression in Human Bronchial Epithelial Cells BEAS-2B | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
TSUCHIYA, Yutaka
× TSUCHIYA, Yutaka× WAKABAYASHI, Aya× MATSUKURA, Satoshi× OSAKABE, Yuki× SEKIGUCHI, Ayaka× INOUE, Daisuke× KAKIUCHI, Yusuke× FUNAKI, Toshitaka× YAMAZAKI, Yohei× TAKAYASU, Hiromi× TATENO, Hidetsugu× KATO, Eisuke× HAYASHI, Makoto× ISHII, Gen× YAMAGUCHI, Fumihiro× KOKUBU, Fumio |
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書誌情報 |
The Showa University journal of medical sciences 巻 28, 号 2, p. 101-111, 発行日 2016-06 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Endothelin-1 is a peptide with many functions including bronchoconstriction and the stimulation of fibroblasts, and myofibroblasts, and airway smooth muscle cell proliferation. These functions are related to airway remodeling and endothelin-1 is known to be upregulated in the epithelium of patients with severe asthma. We thus sought to elucidate the mechanisms underlying endothelin-1 expression in bronchial epithelial cells in vitro. The human bronchial epithelial cell line BEAS-2B was grown in culture and then treated with tumor necrosis factor-alpha (TNF-α), interleukin-4 (IL-4), interleukin-13 (IL-13), and transforming growth factor-beta (TGF-β). Expression of endothelin-1 mRNA and protein was quantified by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. We also repressed expression of the key transcription factor in the pathogenesis of severe asthma, nuclear factor-kappa B (NF-κB), using small interfering RNA (siRNA). TNF-α and TGF-β significantly increased the release of endothelin-1 protein into the culture medium of BEAS-2B cells at 24 h after treatment compared to untreated cells; however, the Th2 cytokines, IL-4 and IL-13, had no effect. Endothelin-1 mRNA expression was also upregulated by TNF-α and TGF-β with a peak time point at 4 h after stimulation. Finally, the combination of TNF-α and TGF-β synergistically increased both endothelin-1 protein secretion and mRNA expression, and this upregulation was significantly suppressed in cells transfected with siRNA to repress NF-κB expression. TNF-α and TGF-β synergistically upregulate the expression of endothelin-1 in human bronchial epithelial cells, possibly via the activity of NF-κB. Our findings thus suggest NF-κBa as a potential therapeutic target for the regulation of airway remodeling. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.28.101 | |||||
出版者 | ||||||
出版者 | Showa University Society | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |