{"created":"2023-05-15T11:21:53.287755+00:00","id":547,"links":{},"metadata":{"_buckets":{"deposit":"7c2cbeb1-8a0f-4af9-b0fc-ffc5bfddf52d"},"_deposit":{"created_by":1,"id":"547","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"547"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000547","sets":["1:51:64:66"]},"author_link":["2799","2808","2805","2802","2807","2810","2797","2801","2803","2804","2809","2795","2796","2800","2798","2806"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"111","bibliographicPageStart":"101","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Endothelin-1 is a peptide with many functions including bronchoconstriction and the stimulation of fibroblasts, and myofibroblasts, and airway smooth muscle cell proliferation. These functions are related to airway remodeling and endothelin-1 is known to be upregulated in the epithelium of patients with severe asthma. We thus sought to elucidate the mechanisms underlying endothelin-1 expression in bronchial epithelial cells in vitro. The human bronchial epithelial cell line BEAS-2B was grown in culture and then treated with tumor necrosis factor-alpha (TNF-α), interleukin-4 (IL-4), interleukin-13 (IL-13), and transforming growth factor-beta (TGF-β). Expression of endothelin-1 mRNA and protein was quantified by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. We also repressed expression of the key transcription factor in the pathogenesis of severe asthma, nuclear factor-kappa B (NF-κB), using small interfering RNA (siRNA). TNF-α and TGF-β significantly increased the release of endothelin-1 protein into the culture medium of BEAS-2B cells at 24 h after treatment compared to untreated cells; however, the Th2 cytokines, IL-4 and IL-13, had no effect. Endothelin-1 mRNA expression was also upregulated by TNF-α and TGF-β with a peak time point at 4 h after stimulation. Finally, the combination of TNF-α and TGF-β synergistically increased both endothelin-1 protein secretion and mRNA expression, and this upregulation was significantly suppressed in cells transfected with siRNA to repress NF-κB expression. TNF-α and TGF-β synergistically upregulate the expression of endothelin-1 in human bronchial epithelial cells, possibly via the activity of NF-κB. Our findings thus suggest NF-κBa as a potential therapeutic target for the regulation of airway remodeling.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.28.101"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380 "}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TSUCHIYA, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WAKABAYASHI, Aya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUKURA, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OSAKABE, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SEKIGUCHI, Ayaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"INOUE, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAKIUCHI, Yusuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUNAKI, Toshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAZAKI, Yohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKAYASU, Hiromi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TATENO, Hidetsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KATO, Eisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HAYASHI, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ISHII, Gen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAGUCHI, Fumihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KOKUBU, Fumio"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S28_101.pdf","filesize":[{"value":"443.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S28_101.pdf","url":"https://showa.repo.nii.ac.jp/record/547/files/S28_101.pdf"},"version_id":"508ce9f3-83ec-4125-b028-53e0a970e61f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta Synergistically Upregulate Endothelin-1 Expression in Human Bronchial Epithelial Cells BEAS-2B ","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta Synergistically Upregulate Endothelin-1 Expression in Human Bronchial Epithelial Cells BEAS-2B "}]},"item_type_id":"10002","owner":"1","path":["66"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-02-14"},"publish_date":"2017-02-14","publish_status":"0","recid":"547","relation_version_is_last":true,"title":["Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta Synergistically Upregulate Endothelin-1 Expression in Human Bronchial Epithelial Cells BEAS-2B "],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:43:31.518700+00:00"}