| Item type |
学内発行雑誌 / Departmental Bulletin Paper(1) |
| 公開日 |
2016-05-13 |
| タイトル |
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|
タイトル |
Development of a New Bioartificial Liver Support System Using a Radial-flow Bioreactor |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ |
departmental bulletin paper |
| 著者 |
AOKI, Takeshi
MURAKAMI, Masahiko
YASUDA, Daisuke
KOIZUMI, Tomotake
JIN, Zhenghao
FUJIMORI, Akira
KUSANO, Tomokazu
MATSUDA, Kazuhiro
YAMADA, Kosuke
NOGAKI, Koji
WADA, Yusuke
ENAMI, Yuta
MITAMURA, Keitaro
GOTO, Satoru
YOSHITAKE, Osamu
WATANABE, Makoto
OTSUKA, Koji
KUSANO, Mitsuo
|
| 書誌情報 |
The Showa University journal of medical sciences
巻 27,
号 3,
p. 155-165,
発行日 2015-09
|
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
There is an increasing number of patients with severe liver disease that requires whole organ transplantation or living-related split liver transplantation. This has resulted in a shortage of donor organs, which is particularly problematic and still awaits resolution. Bioartificial liver (BAL) support systems have been developed with the aim of supporting patients with life-threatening liver disease until their liver recovers. Here, we describe a high performance three-dimensional rat hepatocyte culture system using a radial-flow bioreactor (RFB) with a polyvinyl alcohol (PVA) membrane as a small-scale BAL support system. Hepatocytes from male Sprague-Dawley rat livers were isolated and divided into two groups as follows. Group A: isolated hepatocytes were maintained in culture medium as controls; and group B: isolated hepatocytes were injected into the medium chamber of the RFB-PVA culture system. Sampling was carried out every 48 h to analyze the concentrations of ammonia and albumin in the medium. Light and electron microscopic examination of hepatocytes explanted from the PVA membrane was also performed. Albumin production and urea synthesis by cells in group B were both significantly higher than in group A. Hematoxylin-Eosin staining of the cells in group B showed that three-dimensional cell masses were attached to the PVA membrane. It also showed that the cells were stably proliferating in the porous spaces of the PVA. Scanning electron microscopic images of group B also showed clusters of hepatocytes attached to the PVA membrane. Hepatocyte clusters growing in the RFB-PVA culture system retained their biological function and were stable in the porous spaces of the PVA membrane. This cell culture system may be useful for the development of new BAL support systems. |
| DOI |
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関連タイプ |
isIdenticalTo |
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関連識別子 |
10.15369/sujms.27.155 |
| 出版者 |
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出版者 |
Showa University Society |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0915-6380 |
| 出版タイプ |
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出版タイプ |
VoR |
S27_155.pdf (529.8 kB)
