WEKO3
アイテム
{"_buckets": {"deposit": "11d6cf62-6af7-4221-a0ac-ffcb952a5bc6"}, "_deposit": {"created_by": 1, "id": "1163", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1163"}, "status": "published"}, "_oai": {"id": "oai:showa.repo.nii.ac.jp:00001163", "sets": ["146"]}, "author_link": ["6224", "6222", "6220", "6218", "6221", "6219", "6223"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "81", "bibliographicPageStart": "71", "bibliographicVolumeNumber": "2", "bibliographic_titles": [{"bibliographic_title": "昭和大学薬学雑誌"}]}]}, "item_10002_description_6": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Nicotine, main constituent of tabaco, is known as a nicotinic acetylcholine receptor (nAChR) agonist and increases cognitive performance. 3-(2,4-dimethoxybenzylidene)-anabaseine (GTS-21) is derived from the marine worm toxin, anabaseine, and is alpha7-selective nAChR (D7-nAChR) agonist. Both nicotine and GTS-21 were expected as therapeutic agents of Alzheimer’s disease. Several studies showed that nicotine and GTS-21 protected neuron by activating nAChR, especially D7-nAChR. It has been reported that D7-nAChR has been shown to be an essential regulator of inflammation. The purpose of this study is to examine the neuroprotective and anti-inflammatory effects of nicotine and GTS-21 using organotypic hippocampal slice cultures. Kainic acid (KA, 5-50µM) induced concentration- and time-dependent neuronal cell death in the hippocampal organotypic slice cultures. The pretreatment with nicotine and GTS-21 tended to decrease in KA toxicity. In a CA3 area-specific analysis, pretreatment with nicotine resulted in significant inhibition of KA-induced neurotoxicity. The results suggest that nicotine may protect KA-induced neuronal cell death via D7-nAChR. We also examined anti-inflammatory effects of nicotine and GTS-21. Hippocampal slices were pretreated with nicotine or GTS-21, and then treated with lipopolysaccharide (LPS). LPS treatment induced concentration-dependent increases in TNFD and IL-1E gene expressions. LPS-induced TNFD gene expression, but not IL-1E was suppressed by GTS-21 pretreatment. These results suggest that D7-nAChR might be involved in the microglia activation towards a neuroprotective role by suppressing inflammatory cytokine.", "subitem_description_type": "Abstract"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Showa University School of Pharmacy"}]}, "item_10002_source_id_11": {"attribute_name": "NCID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12464955", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1884-7854", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_text_26": {"attribute_name": "アイテムタイプ", "attribute_value_mlt": [{"subitem_text_value": "学内発行雑誌 / Departmental Bulletin Paper"}]}, "item_10002_text_27": {"attribute_name": "POS_INDEX", "attribute_value_mlt": [{"subitem_text_value": "学内発行雑誌/昭和大学薬学雑誌/2巻(2011)/1号"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ITOH, Yoshitsugu"}], "nameIdentifiers": [{"nameIdentifier": "6218", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TANAKA, Sachiko"}], "nameIdentifiers": [{"nameIdentifier": "6219", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAKAHASHI, Sayaka"}], "nameIdentifiers": [{"nameIdentifier": "6220", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MORIKWA, Chikara"}], "nameIdentifiers": [{"nameIdentifier": "6221", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NUMAZAWA, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "6222", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAIZAKI, Asuka"}], "nameIdentifiers": [{"nameIdentifier": "6223", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YOSHIDA, Takemi"}], "nameIdentifiers": [{"nameIdentifier": "6224", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-26"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "P2_71.pdf", "filesize": [{"value": "1.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1900000.0, "url": {"label": "P2_71.pdf", "url": "https://showa.repo.nii.ac.jp/record/1163/files/P2_71.pdf"}, "version_id": "5ea984e4-22ee-4e55-adf9-457400ecf406"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures."}]}, "item_type_id": "10002", "owner": "1", "path": ["146"], "permalink_uri": "https://showa.repo.nii.ac.jp/records/1163", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-02-08"}, "publish_date": "2016-02-08", "publish_status": "0", "recid": "1163", "relation": {}, "relation_version_is_last": true, "title": ["Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures."], "weko_shared_id": -1}
Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures.
https://showa.repo.nii.ac.jp/records/1163
https://showa.repo.nii.ac.jp/records/11631c037a8a-313a-4d0f-96ed-1c6960759242
名前 / ファイル | ライセンス | アクション |
---|---|---|
P2_71.pdf (1.9 MB)
|
|
Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-02-08 | |||||
タイトル | ||||||
タイトル | Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
ITOH, Yoshitsugu
× ITOH, Yoshitsugu× TANAKA, Sachiko× TAKAHASHI, Sayaka× MORIKWA, Chikara× NUMAZAWA, Satoshi× KAIZAKI, Asuka× YOSHIDA, Takemi |
|||||
書誌情報 |
昭和大学薬学雑誌 巻 2, 号 1, p. 71-81, 発行日 2011-06 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Nicotine, main constituent of tabaco, is known as a nicotinic acetylcholine receptor (nAChR) agonist and increases cognitive performance. 3-(2,4-dimethoxybenzylidene)-anabaseine (GTS-21) is derived from the marine worm toxin, anabaseine, and is alpha7-selective nAChR (D7-nAChR) agonist. Both nicotine and GTS-21 were expected as therapeutic agents of Alzheimer’s disease. Several studies showed that nicotine and GTS-21 protected neuron by activating nAChR, especially D7-nAChR. It has been reported that D7-nAChR has been shown to be an essential regulator of inflammation. The purpose of this study is to examine the neuroprotective and anti-inflammatory effects of nicotine and GTS-21 using organotypic hippocampal slice cultures. Kainic acid (KA, 5-50µM) induced concentration- and time-dependent neuronal cell death in the hippocampal organotypic slice cultures. The pretreatment with nicotine and GTS-21 tended to decrease in KA toxicity. In a CA3 area-specific analysis, pretreatment with nicotine resulted in significant inhibition of KA-induced neurotoxicity. The results suggest that nicotine may protect KA-induced neuronal cell death via D7-nAChR. We also examined anti-inflammatory effects of nicotine and GTS-21. Hippocampal slices were pretreated with nicotine or GTS-21, and then treated with lipopolysaccharide (LPS). LPS treatment induced concentration-dependent increases in TNFD and IL-1E gene expressions. LPS-induced TNFD gene expression, but not IL-1E was suppressed by GTS-21 pretreatment. These results suggest that D7-nAChR might be involved in the microglia activation towards a neuroprotective role by suppressing inflammatory cytokine. | |||||
出版者 | ||||||
出版者 | Showa University School of Pharmacy | |||||
ISSN | ||||||
収録物識別子 | 1884-7854 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |