{"created":"2023-05-15T11:22:32.445857+00:00","id":1163,"links":{},"metadata":{"_buckets":{"deposit":"11d6cf62-6af7-4221-a0ac-ffcb952a5bc6"},"_deposit":{"created_by":1,"id":"1163","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"1163"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00001163","sets":["1:54:145:146"]},"author_link":["6224","6222","6220","6218","6221","6219","6223"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"81","bibliographicPageStart":"71","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"昭和大学薬学雑誌"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nicotine, main constituent of tabaco, is known as a nicotinic acetylcholine receptor (nAChR) agonist and increases cognitive performance. 3-(2,4-dimethoxybenzylidene)-anabaseine (GTS-21) is derived from the marine worm toxin, anabaseine, and is alpha7-selective nAChR (D7-nAChR) agonist. Both nicotine and GTS-21 were expected as therapeutic agents of Alzheimer’s disease. Several studies showed that nicotine and GTS-21 protected neuron by activating nAChR, especially D7-nAChR. It has been reported that D7-nAChR has been shown to be an essential regulator of inflammation. The purpose of this study is to examine the neuroprotective and anti-inflammatory effects of nicotine and GTS-21 using organotypic hippocampal slice cultures. Kainic acid (KA, 5-50µM) induced concentration- and time-dependent neuronal cell death in the hippocampal organotypic slice cultures. The pretreatment with nicotine and GTS-21 tended to decrease in KA toxicity. In a CA3 area-specific analysis, pretreatment with nicotine resulted in significant inhibition of KA-induced neurotoxicity. The results suggest that nicotine may protect KA-induced neuronal cell death via D7-nAChR. We also examined anti-inflammatory effects of nicotine and GTS-21. Hippocampal slices were pretreated with nicotine or GTS-21, and then treated with lipopolysaccharide (LPS). LPS treatment induced concentration-dependent increases in TNFD and IL-1E gene expressions. LPS-induced TNFD gene expression, but not IL-1E was suppressed by GTS-21 pretreatment. These results suggest that D7-nAChR might be involved in the microglia activation towards a neuroprotective role by suppressing inflammatory cytokine.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University School of Pharmacy"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1884-7854"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ITOH, Yoshitsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANAKA, Sachiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKAHASHI, Sayaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MORIKWA, Chikara"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NUMAZAWA, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAIZAKI, Asuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YOSHIDA, Takemi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"P2_71.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"P2_71.pdf","url":"https://showa.repo.nii.ac.jp/record/1163/files/P2_71.pdf"},"version_id":"5ea984e4-22ee-4e55-adf9-457400ecf406"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures."}]},"item_type_id":"10002","owner":"1","path":["146"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-02-08"},"publish_date":"2016-02-08","publish_status":"0","recid":"1163","relation_version_is_last":true,"title":["Alpha7 nicotinic ACh receptor mediated neuroprotective action by nicotine and GTS-21: An approach by the hippocampal organotypic slice cultures."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T12:52:28.393091+00:00"}