{"created":"2023-05-15T11:22:04.986173+00:00","id":737,"links":{},"metadata":{"_buckets":{"deposit":"71f20087-6f78-4459-b2d0-3886ba171e7c"},"_deposit":{"created_by":1,"id":"737","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"737"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000737","sets":["1:51:94:96"]},"author_link":["4290","4294","4293","4292","4289","4288","4287","4291"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-06"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"115","bibliographicPageStart":"105","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The ryanodine receptor type 1 (RyR1) is capable of homotetrameric assembly to form a Ca2+ release channel at intracellular Ca2+ storage sites such as endoplasmic reticulum (ER). The mRNA transcript encoding full-length RyR1 is approximately 16kb and is mainly distributed in excitable cells. A 2.4-kb mRNA splice variant from the 3'-terminal region of the RyR1 gene coexists specifically in brain together with the full-length form, although the functions of this brain-specific splice variant remain unclear. To investigate the short form of RyR1 in intracellular Ca2+ signaling in brain at the cellular level, we established an experimental system whereby the green fluorescent protein (GFP) -tagged brain-specific variant of RyR1 is coexpressed with the full-length protein in the same cell. Both forms of RyR1 were localized in the ER. Caffeine-induced Ca2+-release activities in cells expressing both the brain-specific and full-length RyR1 were reduced compared to cells expressing only the full-length form of RyR1. These results suggested that coexpression of the brain-specific splice variant of RyR1 with its full-length counterpart modulates intracellular Ca2+ signaling by acting as a dominant-negative subunit of the Ca2+ release channel in a tissue-specific fashion.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa Medical Association and Showa University"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.22.105"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HAYASHI, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OYAMADA, Hideto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAZAWA, Toshiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MURAYAMA, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUOKA, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHBA, Keiichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKANO, Masahide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OGUCHI, Katsuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S22_105.pdf","filesize":[{"value":"524.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S22_105.pdf","url":"https://showa.repo.nii.ac.jp/record/737/files/S22_105.pdf"},"version_id":"56a4ae04-2ba7-4efd-8014-2caca703a847"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"The Role of a Brain-specific Splice Variant of Ryanodine Receptor Type 1","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Role of a Brain-specific Splice Variant of Ryanodine Receptor Type 1"}]},"item_type_id":"10002","owner":"1","path":["96"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-12-19"},"publish_date":"2016-12-19","publish_status":"0","recid":"737","relation_version_is_last":true,"title":["The Role of a Brain-specific Splice Variant of Ryanodine Receptor Type 1"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:36:53.077237+00:00"}