{"created":"2023-05-15T11:21:59.666890+00:00","id":655,"links":{},"metadata":{"_buckets":{"deposit":"50fcab60-1247-4206-9c12-36ff68ca0a8a"},"_deposit":{"created_by":1,"id":"655","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"655"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000655","sets":["1:51:79:80"]},"author_link":["3684","3679","3683","3678","3680","3682","3681"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"27","bibliographicPageStart":"19","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Silencing of gene expression by RNA interference (RNAi) has become a widely used tool for assessing gene function in a fast and easy manner. An important advance in the RNAi field was the discovery that plasmid-based short hairpin RNA transcription can substitute for synthetic small interference RNAs both in vitro and in vivo. The constitutive knockdown of gene expression by RNAi can limit the scope of experiments, especially if the inhibition of genes leads to cell lethality, which prevents in vivo functional analysis. We have generated double-conditional short hairpin RNA from a single vector that can regulate its own transcription by a combination of tetracycline-inducible and Cre-loxP systems. This vector will be useful for the control of short hairpin RNA expression in a spatially-regulated manner by Cre recombinase as well as a temporally-regulated manner by tetracycline induction. These control features provide for the specific silencing of genes to facilitate functional genetic analysis in mammals in vivo.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.25.19"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MATSUOKA, Tomoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OYAMADA, Hideto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKANO, Masahideo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SOMEI, Masayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOMOYORI, Eiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KONDO, Yasuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OGUCHI, Katsuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S25_19.pdf","filesize":[{"value":"878.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S25_19.pdf","url":"https://showa.repo.nii.ac.jp/record/655/files/S25_19.pdf"},"version_id":"dbb4af7d-036e-4c60-9939-edc4ba764d17"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Construction of a Novel Single Double-Conditional shRNA Expression Vector","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Construction of a Novel Single Double-Conditional shRNA Expression Vector"}]},"item_type_id":"10002","owner":"1","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-11-22"},"publish_date":"2016-11-22","publish_status":"0","recid":"655","relation_version_is_last":true,"title":["Construction of a Novel Single Double-Conditional shRNA Expression Vector"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:39:28.508638+00:00"}