{"created":"2023-05-15T11:21:49.435107+00:00","id":484,"links":{},"metadata":{"_buckets":{"deposit":"c81196be-82a9-44ee-8d12-6c0f0230fcd1"},"_deposit":{"created_by":1,"id":"484","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"484"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000484","sets":["1:51:56:57"]},"author_link":["2311","2314","2309","2310","2312","2315","2313"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"85","bibliographicPageStart":"73","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Photokeratitis is a disease in which the ocular surface is directly affected by oxidative stress caused by exposure to ultraviolet （UV） light and oxygen. It is speculated that the production of free radicals and reactive oxygen species （ROS） is caused by UV-induced cytotoxicity. Recent studies have reported that catechins have antioxidant, antiallergic, antitumor, and antibacterial effects. The aim of our study was to investigate the mechanism of UV-induced cytotoxicity in cultured human corneal epithelial （HCE-T） cells and evaluate the protective effects of the catechins, （?）-epigallocatechin gallate （EGCG） and （?）-epigallocatechin 3-O-（3-O-methyl） gallate （EGCG3”Me）, on apoptosis. HCE-T cells were UV irradiated at 312nm （4.94mW/cm2, 296mJ/cm2）. EGCG and EGCG3”Me were dissolved in methanol and adjusted to 5, 10, or 20?M. Absorption was measured from 250 to 400nm. EGCG and EGCG3”Me were pre-incubated for 1 hr. After UV irradiation, membrane lipid peroxide, tumor necrosis factor （TNF）-α production, ROS generation, caspase-3 and -8 activities, mitochondrial membrane potential, and cytochrome c levels were measured. Both EGCG and EGCG3”Me had UV absorption, and increased with concentration dependently. The increases in the levels of membrane lipid hyperoxidation, activation of caspase-3 and -8, production of TNF-α and ROS were found, by UV irradiation, to be significant. But these levels were significantly decreased by pretreatment with EGCG and EGCG3”Me. There were no changes in mitochondrial membrane potential and cytoplasmic cytochrome c levels after UV irradiation. Oxidative stress occurs early near the cell membrane in response to UV irradiation. As a result, TNF-α is induced, leading to apoptosis mainly through caspase-8 activation. Conversely, EGCG and EGCG3”Me absorb UV light directly and inhibit lipid peroxidation in the cell membrane. Catechins inhibit the apoptosis cascade by inactivating caspase-3 and caspase-8.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.30.73"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380 "}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TOJU, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UDAKA, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUJI, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OYAMADA, Hideto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIZAKI, Junichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOMOYORI, Eiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIUCHI, Yuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S30_73.pdf","filesize":[{"value":"947.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S30_73.pdf","url":"https://showa.repo.nii.ac.jp/record/484/files/S30_73.pdf"},"version_id":"dda461ba-7219-4d9d-9e93-4e672a152e2e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Suppressive Effects of Catechins in UV-Induced Cytotoxicity of Human Corneal Epithelial Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Suppressive Effects of Catechins in UV-Induced Cytotoxicity of Human Corneal Epithelial Cells"}]},"item_type_id":"10002","owner":"1","path":["57"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-20"},"publish_date":"2018-11-20","publish_status":"0","recid":"484","relation_version_is_last":true,"title":["Suppressive Effects of Catechins in UV-Induced Cytotoxicity of Human Corneal Epithelial Cells"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:45:43.364025+00:00"}