{"created":"2023-05-15T11:21:49.365059+00:00","id":483,"links":{},"metadata":{"_buckets":{"deposit":"9e08af70-55b2-418b-821c-631099826cdb"},"_deposit":{"created_by":1,"id":"483","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"483"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00000483","sets":["1:51:56:57"]},"author_link":["2308","2306","2305","2304","2301","2302","2307","2303"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"72","bibliographicPageStart":"63","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Osteoarthritis （OA） is a degenerative disease that occurs in joints throughout the body and includes various concomitant pathologies due to possible mechanical stress, such as destruction of cartilage, hyperplasic changes, and synovial inflammation. However, there have been few studies on the mechanical stress that is the basic cause of OA. Our goal was to establish an OA model at the cellular level, by measuring inflammatory cytokines and cartilage destruction markers that are induced after a mechanical stress load. Using a human synovial sarcoma cell line （SW982 cells）, we provided two types of mechanical stress load for 48hr: shaking stress （amplitude 2mm, speed range 1,000rpm）, and the addition of hydroxyapatite （5?g/ml） into the culture medium. Then we measured the phosphorylation activity of nuclear factor （NF）-κB transcription factor in the cell lysate, and the levels of tumor necrosis factor （TNF）-α and interleukin （IL）-6 as inflammatory cytokines, and the level of matrix metalloproteinase （MMP）-3 as a cartilage destruction marker, released in the medium. Shaking stress significantly induced phosphorylation of NF-κB and production of TNF-α, compared with untreated controls. On the other hand, hydroxyapatite stress only increased production of TNF-α. Both stresses together significantly induced phosphorylation of NF-κB and production of TNF-α, IL-6 and MMP-3 rather than a single stress load. In this study, markers related to inflammation and cartilage destruction （IL-6, TNF-α, and MMP-3） significantly increased. Therefore, we suggest that the mechanical stress load conditions used in this study might be useful as an OA model.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.30.63"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380 "}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KOBAYASHI, Yoshito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UDAKA, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIRAKO, Haruna"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KATO, Miki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KUDO, Yoshiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ICHIMURA, Nana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUJI, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIUCHI, Yuji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-26"}],"displaytype":"detail","filename":"S30_63.pdf","filesize":[{"value":"465.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S30_63.pdf","url":"https://showa.repo.nii.ac.jp/record/483/files/S30_63.pdf"},"version_id":"77469a1c-4a89-41ce-ab5b-41bab653b201"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Establishment of a Mechanical Stress Load Arthritis Model in a Human Synovial Sarcoma Cell Line","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment of a Mechanical Stress Load Arthritis Model in a Human Synovial Sarcoma Cell Line"}]},"item_type_id":"10002","owner":"1","path":["57"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-08-23"},"publish_date":"2018-08-23","publish_status":"0","recid":"483","relation_version_is_last":true,"title":["Establishment of a Mechanical Stress Load Arthritis Model in a Human Synovial Sarcoma Cell Line"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:45:45.816434+00:00"}