{"created":"2023-05-15T11:24:07.987383+00:00","id":3856,"links":{},"metadata":{"_buckets":{"deposit":"da72b9da-7f32-43c5-9ac5-a7a967bca082"},"_deposit":{"created_by":1,"id":"3856","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"3856"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00003856","sets":["1:51:308:338"]},"author_link":["12991","12989","12998","12999","13000","12992","12997","12996","13001","12994","12995","12993","12990","13002"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-12"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"132","bibliographicPageStart":"124","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The cytokine interleukin-34 （IL-34） was recently described. However, its role in the lungs is not well understood. IL-34 binds to the colony stimulating factor-1 receptor, thereby enhancing tissue macrophage maturation and differentiation. Macrophages are essential to the airway inflammation process and acute lung injury （ALI）. This study aimed to evaluate the role of IL-34 in ALI establishment. C57BL/6 male mice were stimulated intratracheally with lipopolysaccharide （LPS） and sacrificed on day 1, 3, 5, or 7. Additionally, the mice were treated with an anti-IL-34 antibody intranasally before LPS stimulation. The bronchoalveolar lavage fluid （BALF） and lung tissues were collected. The cells of the human peripheral blood monocyte cell line THP-1 and the human airway epithelial cell line BEAS-2B were cultured with LPS in vitro. The total cell number in BALF was higher in the LPS-stimulated mice than in the control mice. The BALF IL-34 level was significantly elevated in BALF on days 1 and 3. IL-34 expression was detected in the pulmonary epithelium in the LPS-stimulated mice on day 1. Anti-IL-34 antibody suppressed the number of macrophages in BALF. IL-34 blockade resulted in pulmonary fibrosis reduction in LPS-stimulated mice on day 5. LPS stimulation in vitro induced the production of tumor necrosis factor-α （TNF-α） in THP-1 cells. Furthermore, TNF-α stimulation induced the IL-34 production in BEAS-2B cells. These results suggest that IL-34 induction in the epithelial cells may enhance pulmonary inflammation and fibrosis in the murine model of LPS-induced acute lung injury.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.33.124"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2185-0968"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"EBATO, Takaya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHTA, Shin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAGUCHI, Munehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIKUNI, Hatsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IKEDA, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"JINNO, Megumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIRAI, Kuniaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIYATA, Yoshito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"INOUE, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HOMMA, Tetsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAMOTO, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SUZUKI, Shintaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANAKA, Akihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAGARA, Hironori"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-11"}],"displaytype":"detail","filename":"S33_124.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S33_124.pdf","url":"https://showa.repo.nii.ac.jp/record/3856/files/S33_124.pdf"},"version_id":"5b95effb-659b-43f4-94e4-fa91196b5b72"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Interleukin-34 induces pulmonary inflammation in a murine model of lipopolysaccharide-induced acute lung injury","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Interleukin-34 induces pulmonary inflammation in a murine model of lipopolysaccharide-induced acute lung injury"}]},"item_type_id":"10002","owner":"1","path":["338"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-11"},"publish_date":"2022-01-11","publish_status":"0","recid":"3856","relation_version_is_last":true,"title":["Interleukin-34 induces pulmonary inflammation in a murine model of lipopolysaccharide-induced acute lung injury"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:02:30.793050+00:00"}