@article{oai:showa.repo.nii.ac.jp:00003839,
 author = {TAKAHASHI, Rei and IWAMOTO, Sanju and TANIOKA, Toshihiro and MAEDA, Kohei},
 issue = {3},
 journal = {The Showa University journal of medical sciences},
 month = {2021-09, 2021-09-22},
 note = {Monocyte-derived Langerhans cell-like dendritic cells （Mo-LCs） are involved in epidermal disorders such as psoriasis in murine models. However, the roles of Mo-LCs in the pathogenesis of psoriasis in humans remain unclear. Also, the contribution of notch ligand delta-like 1 （DLL-1）, expressed on keratinocytes, to Mo-LC functions requires clarification. Here, we established a new method of stimulating Mo-LCs derived from CD14＋ monocytes with immobilized human DLL-1 to generate induced Mo-LCs （DI（＋）Mo-LCs）. The DI（＋）Mo-LCs were compared to the dendritic cells derived from monocytes （Mo-DCs） cultured with interleukin-4 （IL-4） and granulocyte-macrophage colony-stimulating factor （GM-CSF）, and M1 macrophages （Mφ） derived from monocytes cultured with GM-CSF. The DI（＋）Mo-LCs were found to produce significant amounts of IL15, IL23A, and interferon-β （IFNB1） in response to the Toll-like receptor （TLR）3 agonist Polyinosinic-polycytidylic acid （Poly（I:C）） or TLR4 agonist lipopolysaccharide （LPS） despite their low expression of tumor necrosis factor （TNF）. In conclusion, we have established a new method to generate DI（＋）Mo-LCs. We have also discovered that DI（＋）Mo-LCs have a unique capacity for producing IL15 and IL23A, which are related to the pathogenesis of psoriasis. Our data contribute to a better understanding of the roles of Mo-LCs in epidermal defense and pathogenesis.},
 pages = {67--73},
 title = {A profile of pro-inflammatory cytokine expression in human Delta-1-induced monocyte-derived Langerhans cell-like dendritic cells after stimulation with Toll-like receptor ligands},
 volume = {33},
 year = {}
}