{"created":"2023-05-15T11:23:43.722134+00:00","id":3461,"links":{},"metadata":{"_buckets":{"deposit":"d604be47-3e27-4d2f-a1b0-95a6d9449ea0"},"_deposit":{"created_by":1,"id":"3461","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"3461"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00003461","sets":["1:51:271:279"]},"author_link":["11723","11722","11724"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"145","bibliographicPageStart":"135","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although antigen-specific T helper （Th） cells are developed from naive T cells, human Th17 cells are not derived from naive CD4+ T cells unlike murine cells. Therefore, the source of human Th17 cells has remained unresolved. In this study, we assessed the early differentiation pathway of human Th17 cells from CD31+ thymic naive T cells into stem cell memory CCR6+ Th17 precursors and the regulation of this process by cytokines. Peripheral blood mononuclear cells were isolated from healthy volunteers. We found that only CD31- CCR6+ naive type CD4+ T cells had the ability to produce IL-17A in response to Th17-inducing stimuli. A cell tracking assay using CD31+ CCR6- cells labeled with carboxyfluorescein diacetate succinimidyl ester revealed that CD31- CCR6+ Th17 precursors were derived from CD31+ CCR6- thymic naive T cells. CD31 is known to suppress IL-17 production by interfering with downstream T cell receptor （TCR） signaling molecules including Lck, which is essential for IL-17 production. The inactive form of Lck was much higher in CD31+ T cells than CD31- T cells after TCR stimulation. In experiments of cytokine-mediated modulation of Th17 cell differentiation, IL-4 suppressed the conversion of CD31+ CCR6- naive T cells into CD31- CCR6+ Th17 precursors by upregulating CD31 expression and suppressing CCR6 expression. In conclusion, CD31- CCR6+ Th17 precursors could be sourced from CD31+ CCR6- naive T cells, and IL-4 regulated the early Th17 differentiation. Our findings provide novel insights into the regulation of differentiation of naive CD4+ T cells into Th17 cells in humans. Furthermore, our results may provide hints for further elucidation of the differentiation process of Th17 cells and of the pathology of Th17 cell-related diseases.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.32.135"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2185-0968"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MAEDA, Kohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANIOKA, Toshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IWAMOTO, Sanju"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-08-19"}],"displaytype":"detail","filename":"S32_135.pdf","filesize":[{"value":"840.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S32_135.pdf","url":"https://showa.repo.nii.ac.jp/record/3461/files/S32_135.pdf"},"version_id":"b4f0d860-bcc1-4416-bc97-185a22c4dd15"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Regulatory Effect of IL-4 on Early Th17 Differentiation from Naive T Cells into Stem Cell Memory Th17 Precursors via Modulation of CD31 and CCR6 Expression","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulatory Effect of IL-4 on Early Th17 Differentiation from Naive T Cells into Stem Cell Memory Th17 Precursors via Modulation of CD31 and CCR6 Expression"}]},"item_type_id":"10002","owner":"1","path":["279"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-19"},"publish_date":"2020-08-19","publish_status":"0","recid":"3461","relation_version_is_last":true,"title":["Regulatory Effect of IL-4 on Early Th17 Differentiation from Naive T Cells into Stem Cell Memory Th17 Precursors via Modulation of CD31 and CCR6 Expression"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:31:10.472696+00:00"}