@article{oai:showa.repo.nii.ac.jp:00002985,
 author = {KUWAHARA, Naota and YAMAGUCHI, Munehiro and TANAKA, Akihiko and OHTA, Shin and UNO, Tomoki and UCHIDA, Yoshitaka and MANABE, Ryo and JINNO, Megumi and HIRAI, Kuniaki and MIYATA, Yoshito and MIZUMA, Hiroko and HOMMA, Tetsuya and YAMAMOTO, Mayumi and YAMAGUCHI, Fumihiro and KUSUMOTO, Sojiro and SUZUKI, Shintaro and OHNISHI, Tsukasa and SAGARA, Hironori},
 issue = {1},
 journal = {The Showa University journal of medical sciences},
 month = {2019-03, 2019-08-09},
 note = {Chlorella extract （CE） has been shown to induce production of T helper-1 cytokines, and regulate serum IgE levels in animal models of asthma. We aimed to evaluate whether CE could inhibit ovalbumin （OVA）-induced airway hyperresponsiveness （AHR） and airway remodeling in a murine model of asthma. Balb/c mice were allocated to four groups: a control group （no OVA exposure, not given CE）, a CE group （no OVA exposure, given CE）, an asthma group （sensitized/challenged with OVA, not given CE） and a CE＋asthma group （sensitized/challenged with OVA, given CE）. In the asthma and CE＋asthma groups, mice were sensitized with OVA on day 0 and day 12, and then challenged with OVA on three consecutive days. In the CE and CE＋asthma groups, the mice were given feed containing 2％ CE. We assessed AHR to methacholine, and analyzed bronchoalveolar lavage fluid （BALF）, serum, lung tissue and spleen cells. Administration of CE was associated with significantly lower AHR in OVA-sensitized and challenged mice. CE administration was also associated with marked reduction of total cells, eosinophils and T helper-2 cytokines （IL-4, IL-5 and IL-13） in BALF. In addition, administration of CE significantly decreased the numbers of periodic acid-Schiff （PAS）-positive cells in OVA-sensitized and challenged mice. Administration of CE also directly suppressed IL-4, IL-5 and IL-13 production in spleen cells of OVA-sensitized and challenged mice. These results indicate that CE can partly prevent AHR and airway remodeling in a murine model of asthma.},
 pages = {1--12},
 title = {Inhibitory Effects of Chlorella Extract on Airway Hyperresponsiveness and Airway Remodeling in a Murine Model of Asthma},
 volume = {31},
 year = {}
}