{"created":"2023-05-15T11:23:21.553734+00:00","id":2953,"links":{},"metadata":{"_buckets":{"deposit":"877aa058-68c7-4544-ba4f-e42e3ab5ba65"},"_deposit":{"created_by":1,"id":"2953","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2953"},"status":"published"},"_oai":{"id":"oai:showa.repo.nii.ac.jp:00002953","sets":["1:51:56:238"]},"author_link":["9964","9963","9965","9969","9966","9970","9961","9962","9967","9960","9971","9968"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-09"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"370","bibliographicPageStart":"359","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"The Showa University journal of medical sciences"}]}]},"item_10002_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Triple-negative breast cancer （TNBC） accounts for 10-15％ of all breast cancer cases and shows a poor prognosis with 30％ distant metastasis. With few specific target molecules and ineffective hormonal and anti-HER2 treatment, an alternative therapeutic method for TNBC is urgently required. Recently, a non-taxane inhibitor of microtubule dynamics called eribulin was developed for breast cancer therapy. Eribulin induces irreversible mitotic mass formation in cancer cells during the G2-M phase, initiating apoptosis; however, the mechanism underlying this eribulin activity remains unclear. We reported previously that exposing non-basal-like （NBL） TNBC cells to eribulin increases miR-195 expression, which in turn decreases the expression of targeted Wnt3a. The present study sought to further clarify the mechanism of this antitumor effect by exploring how eribulin affects Wnt/β - catenin signaling based on miRNA expression changes in TNBC. In an NBL type of human breast cancer cell line （MDA-MB-231 cells）, we compared the expression levels of Wnt/β catenin signaling pathway proteins in cells exposed to an miR-195 mimic （cells transfected with miR-195 and in which Wnt3a expression was suppressed） and in cells exposed to eribulin. Expression levels of Wnt3a, β -catenin, and GSK-3β were measured by ELISA and observed by fluorescence immunostaining. Wnt3a and β -catenin expression was significantly lower and GSK-3β expression was significantly higher in the cells exposed to eribulin and transfected with miR-195 mimic than in the untreated controls, suggesting that eribulin inactivates the Wnt/β -catenin signaling pathway. Therefore, a novel antitumor mechanism of eribulin was determined, whereby eribulin induces high expression of miR-195 to inactivate the Wnt/β -catenin signaling pathway in NBL-type TNBC.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Showa University Society"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.15369/sujms.30.359"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-6380 "}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OKAZAKI, Keinosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SASAKI, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUNODA, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FURUYA, Kanji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUJI, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"UDAKA, Yuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OYAMADA, Hideto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HOSONUMA, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SHIRAKO, Haruna"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YASUMOTO, Taro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIUCHI, Yuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SASAKI, Tadanori"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"S30_359.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S30_359.pdf","url":"https://showa.repo.nii.ac.jp/record/2953/files/S30_359.pdf"},"version_id":"f9b9985f-4878-41c8-9876-096acb246390"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper"}]},"item_title":"Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β - catenin Signaling Pathway in Triple-negative Breast Cancer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β - catenin Signaling Pathway in Triple-negative Breast Cancer"}]},"item_type_id":"10002","owner":"1","path":["238"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-08-05"},"publish_date":"2019-08-05","publish_status":"0","recid":"2953","relation_version_is_last":true,"title":["Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β - catenin Signaling Pathway in Triple-negative Breast Cancer"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T12:46:34.406871+00:00"}