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Optogenetic Stimulation of 5-HT Neurons in the Median Raphe Nucleus Affects Anxiety and Respiration
https://showa.repo.nii.ac.jp/records/3045
https://showa.repo.nii.ac.jp/records/3045dde7942a-f64b-42e4-a3fd-4d05c4d6adc5
名前 / ファイル | ライセンス | アクション |
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S31_263.pdf (673.5 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-11-29 | |||||
タイトル | ||||||
タイトル | Optogenetic Stimulation of 5-HT Neurons in the Median Raphe Nucleus Affects Anxiety and Respiration | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
OKUMA, Naoki
× OKUMA, Naoki× KANAMARU, Mitsuko× MORIYA, Rika× TANAKA, Kenji F.× ARATA, Satoru× WATANABE, Jun× YOSHIKAWA, Akira× IZUMIZAKI, Masahiko |
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書誌情報 |
The Showa University journal of medical sciences 巻 31, 号 3, p. 263-274, 発行日 2019-09 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Anxiety affects respiration, and in turn perturbs the internal environment, although the neuronal systems controlling anxiety-related respiration remain unclear. Recent reports indicate that serotonin(5-HT)neurons in the median raphe nucleus(MRN)enhance anxiety. In the present study, we aimed to clarify the contribution of 5-HT neurons in the MRN to anxiety and respiratory control using mice expressing a channelrhodopsin-2 variant-enhanced yellow fluorescent protein(ChR2 [C128S]-EYFP; a step-function opsin)in the central 5-HT neurons. We applied an optogenetic method to bigenic mice expressing ChR2[C128S]-EYFP in 5-HT neurons and to monogenic mice without such expression. Photostimulation of free-moving mice was performed using a wireless system through an optical fiber pre-inserted above the MRN, and respiratory variables were measured using whole-body plethysmography. Anxiety was evaluated using an elevated-plus maze test. In the bigenic mice, we confirmed ChR2[C128S]-EYFP expression in tryptophan hydroxylase 2(a brain 5-HT synthase)-positive neurons in the raphe nuclei of the mesopontine, such as the MRN and the dorsal raphe nucleus. Blue light illumination to the MRN of the bigenic mice significantly increased respiratory rate and minute ventilation without affecting tidal volume, and significantly decreased the time spent in the open arms of the elevated plus maze without changing distance traveled, compared with monogenic mice. These results suggest that 5-HT neuron activity in the MRN increases anxiety-like behavior without affecting locomotor activity, enhances respiratory rhythm and minute ventilation without changing tidal volume, and can mimic anxiety-related respiratory responses in humans. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.31.263 | |||||
出版者 | ||||||
出版者 | Showa University Society | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |