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アイテム
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The Role of a Brain-specific Splice Variant of Ryanodine Receptor Type 1
https://showa.repo.nii.ac.jp/records/737
https://showa.repo.nii.ac.jp/records/737bc4bac52-79ff-43f7-bb66-7fc8e5d15462
名前 / ファイル | ライセンス | アクション |
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S22_105.pdf (524.3 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-12-19 | |||||
タイトル | ||||||
タイトル | The Role of a Brain-specific Splice Variant of Ryanodine Receptor Type 1 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
HAYASHI, Takahiro
× HAYASHI, Takahiro× OYAMADA, Hideto× YAMAZAWA, Toshiko× MURAYAMA, Takashi× MATSUOKA, Tomoyuki× OHBA, Keiichiro× NAKANO, Masahide× OGUCHI, Katsuji |
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書誌情報 |
The Showa University journal of medical sciences 巻 22, 号 2, p. 105-115, 発行日 2010-06 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The ryanodine receptor type 1 (RyR1) is capable of homotetrameric assembly to form a Ca2+ release channel at intracellular Ca2+ storage sites such as endoplasmic reticulum (ER). The mRNA transcript encoding full-length RyR1 is approximately 16kb and is mainly distributed in excitable cells. A 2.4-kb mRNA splice variant from the 3'-terminal region of the RyR1 gene coexists specifically in brain together with the full-length form, although the functions of this brain-specific splice variant remain unclear. To investigate the short form of RyR1 in intracellular Ca2+ signaling in brain at the cellular level, we established an experimental system whereby the green fluorescent protein (GFP) -tagged brain-specific variant of RyR1 is coexpressed with the full-length protein in the same cell. Both forms of RyR1 were localized in the ER. Caffeine-induced Ca2+-release activities in cells expressing both the brain-specific and full-length RyR1 were reduced compared to cells expressing only the full-length form of RyR1. These results suggested that coexpression of the brain-specific splice variant of RyR1 with its full-length counterpart modulates intracellular Ca2+ signaling by acting as a dominant-negative subunit of the Ca2+ release channel in a tissue-specific fashion. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.22.105 | |||||
出版者 | ||||||
出版者 | Showa Medical Association and Showa University | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |