WEKO3
アイテム
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Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes
https://showa.repo.nii.ac.jp/records/717
https://showa.repo.nii.ac.jp/records/7170b0b1ae1-535d-48bf-86b9-944564dbf6ba
名前 / ファイル | ライセンス | アクション |
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S23_1.pdf (167.3 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-12-17 | |||||
タイトル | ||||||
タイトル | Ultraviolet Action Spectrum and Effect of EPC-K1 on Ultraviolet Radiation-induced Injury in Cultured Normal Human Epidermal Keratinocytes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
AOKI, Kayo
× AOKI, Kayo× NAKANISHI-UEDA, Takako× TSUJI, Mayumi× OKUNO, Tsutomu× SAITO, Yusuke× YASUHARA, Hajime |
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書誌情報 |
The Showa University journal of medical sciences 巻 23, 号 1, p. 1-10, 発行日 2011-03 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study was aimed to determine the ultraviolet (UV: 235-310nm) action spectrum for killing normal human epidermal keratinocytes (NHEK) and to investigate the preventive effect of EPC-K1, a phosphate diester of vitamin C and vitamin E on UV radiation-induced NHEK injury. NHEK were cultured in EpiLife medium supplemented with Human Keratinocyte Growth Supplement Kit. NHEK viability was determined by crystal violet (CV) staining 48 h after the UV irradiation. The mRNA expressions of the C/EBP homologous protein (Chop) transcription factor and endoplasmic reticulum-resident molecular chaperone, Bip, were determined by RT-PCR analyses. UV was especially effective in killing NHEK when applied in the wavelength region of 250-280nm. The minimum exposure dose required to kill 50% of cells (LD50) was 1.64mJ/cm2 at 269nm. At 235 and 310nm, the LD50 for NHEK was 6.62 and 293mJ/cm2, respectively. Irradiation of 660-mJ/cm2 at 310nm significantly decreased the cell viability to 30% of control (without irradiation). The addition of 0.1mM EPC-K1 after irradiation returned the cell viability to 118%. Six hours after the 660-mJ/cm2 irradiation at 310nm, Chop and Bip mRNA levels in NHEK were increased to 487% and 283%, respectively, and were not significantly affected by EPC-K1. Chop and Bip are responsive to ER stress. These results suggested that EPC-K1 exerts a protective effect against UV-induced NHEK injury, and further studies should investigate the molecular mechanism underlying this effect. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.23.1 | |||||
出版者 | ||||||
出版者 | Showa Medical Association and Showa University | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |