WEKO3
アイテム
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Effect of Hydrogen Peroxide and High Glucose on the Glucose Metabolism of Lymphoma-derived U937 Cells
https://showa.repo.nii.ac.jp/records/638
https://showa.repo.nii.ac.jp/records/6380b076d80-ca2a-4b23-993d-3d10e5c793ab
名前 / ファイル | ライセンス | アクション |
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S25_193.pdf (275.1 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-11-18 | |||||
タイトル | ||||||
タイトル | Effect of Hydrogen Peroxide and High Glucose on the Glucose Metabolism of Lymphoma-derived U937 Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
TAKEDA, Yasunori
× TAKEDA, Yasunori× NAKAMURA, Yuya× INAGAKI, Masahiro× TSUJI, Mayumi× HASEGAWA, Hitomi× WATANABE, Masanobu× GOTOH, Hiromichi× GOTO, Yoshikazu× OGUCHI, Katsuji |
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書誌情報 |
The Showa University journal of medical sciences 巻 25, 号 3, p. 193-201, 発行日 2013-09 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Our study aimed to clarify specific oxidative stress and glucose metabolic disorders in hemodialysis patients, by examining hydrogen peroxide (H2O2) - and high glucose-induced oxidative stress, glucose transport and the failure of glycolysis. As an in vitro blood cell model of end-stage renal disease (ESRD) in patients with diabetes, human monocytic U937 cells of malignant lymphoma origin were exposed to high glucose (28.9mM) for 6 days, with 5mM H2O2 added on the last day. The generation of intracellular reactive oxygen species (ROS), glucose levels, lactate levels, AMP-activated protein kinase (AMPK) activity and Glut4 levels were examined. Exposure of U937 cells to H2O2 resulted in a significant increase in intracellular ROS generation and glucose levels. Under high glucose conditions, treatment with H2O2 significantly promoted these actions. In H2O2-induced U937 cells, AMPK activity and Glut4 levels were significantly increased, but lactate and pyruvate levels were significantly decreased. Thus, exposure of U937 cells to H2O2 and a high glucose load promoted an increase in intracellular ROS, and exposure to H2O2 induced increased glucose transport and high intracellular glucose due to reduced glycolytic metabolism. This suggests that reduced glycolytic metabolism might be induced in states of high oxidative stress in hemodialysis patients with diabetes. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.25.193 | |||||
出版者 | ||||||
出版者 | Showa University Society | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |