WEKO3
アイテム
{"_buckets": {"deposit": "f77b6b3a-a58c-44f1-a194-a88863462bbd"}, "_deposit": {"created_by": 1, "id": "602", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "602"}, "status": "published"}, "_oai": {"id": "oai:showa.repo.nii.ac.jp:00000602", "sets": ["77"]}, "author_link": ["3263", "3262", "3261"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "190", "bibliographicPageStart": "181", "bibliographicVolumeNumber": "26", "bibliographic_titles": [{"bibliographic_title": "The Showa University journal of medical sciences"}]}]}, "item_10002_description_6": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Quinolone resistance has been attributed to amino acid mutations in the type II topoisomerases of pathogens. To better understand this mechanism of resistance, we analyzed the molecular epidemiology of levofloxacin-resistant Streptococcus pneumoniae in Japan. We measured the quinolone susceptibility of 668 strains of S. pneumoniae obtained through nationwide surveillance from 2006 to 2008. We also sequenced the quinolone resistance-determining regions (QRDRs) in type II topoisomerases and analyzed the relationship between minimum inhibitory concentration (MIC) and six specific mutations (Ser81 or Glu85 in GyrA, Asp435 in GyrB, Ser79 or Asp83 in ParC, and Asp435 in ParE). Eighteen of the isolated strains (2.7%) showed intermediate susceptibility and resistance to levofloxacin (MIC \u003e 4µg/ml), with no significant difference among years. However, garenoxacin and sitafloxacin showed excellent activity against these strains. Of the 18 strains, 17 (94.4%) showed mutation in QRDRs, while in 12 out of 14 levofloxacin-resistant strains (85.7%) two or more mutations were identified. A single QRDR mutation was found in 3 of 60 levofloxacin-susceptible strains (5%), all of which had an MIC of 2µg/ml. We therefore found a high isolation frequency of levofloxacin-resistant S. pneumonia in Japan over a 2-year period. Furthermore, QRDR mutations were present in 5% of susceptible strains; these were thought to be in the early stages of resistance. In the future, the increasing use of levofloxacin might result in more strain resistance. We therefore suggest that strong quinolones such as garenoxacin and sitafloxacin could be proactively administered to high-risk patients.", "subitem_description_type": "Abstract"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Showa University Society"}]}, "item_10002_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.15369/sujms.26.181", "subitem_relation_type_select": "DOI"}}]}, "item_10002_source_id_11": {"attribute_name": "NCID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10781651", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0915-6380 ", "subitem_source_identifier_type": "ISSN"}]}, "item_10002_text_26": {"attribute_name": "アイテムタイプ", "attribute_value_mlt": [{"subitem_text_value": "学内発行雑誌 / Departmental Bulletin Paper"}]}, "item_10002_text_27": {"attribute_name": "POS_INDEX", "attribute_value_mlt": [{"subitem_text_value": "学内発行雑誌/The Showa University journal of medical sciences/Vol.26(2014)/No.3"}]}, "item_10002_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "SHOJI, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "3261", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAKUMA, Takahiro"}], "nameIdentifiers": [{"nameIdentifier": "3262", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NIKI, Yoshihito"}], "nameIdentifiers": [{"nameIdentifier": "3263", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-26"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "S26_181.pdf", "filesize": [{"value": "173.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 173900.0, "url": {"label": "S26_181.pdf", "url": "https://showa.repo.nii.ac.jp/record/602/files/S26_181.pdf"}, "version_id": "800ccf82-bb3b-4aea-a279-6744d85f18c9"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Molecular Analysis of Levofloxacin-resistant Streptococcus pneumoniae in Japan", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Molecular Analysis of Levofloxacin-resistant Streptococcus pneumoniae in Japan", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "1", "path": ["77"], "permalink_uri": "https://showa.repo.nii.ac.jp/records/602", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-11-20"}, "publish_date": "2015-11-20", "publish_status": "0", "recid": "602", "relation": {}, "relation_version_is_last": true, "title": ["Molecular Analysis of Levofloxacin-resistant Streptococcus pneumoniae in Japan"], "weko_shared_id": 1}
Molecular Analysis of Levofloxacin-resistant Streptococcus pneumoniae in Japan
https://showa.repo.nii.ac.jp/records/602
https://showa.repo.nii.ac.jp/records/6029ff82a43-21d4-4c7d-af47-d18af9a32da6
名前 / ファイル | ライセンス | アクション |
---|---|---|
S26_181.pdf (173.9 kB)
|
|
Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-11-20 | |||||
タイトル | ||||||
タイトル | Molecular Analysis of Levofloxacin-resistant Streptococcus pneumoniae in Japan | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
SHOJI, Hisashi
× SHOJI, Hisashi× TAKUMA, Takahiro× NIKI, Yoshihito |
|||||
書誌情報 |
The Showa University journal of medical sciences 巻 26, 号 3, p. 181-190, 発行日 2014-09 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Quinolone resistance has been attributed to amino acid mutations in the type II topoisomerases of pathogens. To better understand this mechanism of resistance, we analyzed the molecular epidemiology of levofloxacin-resistant Streptococcus pneumoniae in Japan. We measured the quinolone susceptibility of 668 strains of S. pneumoniae obtained through nationwide surveillance from 2006 to 2008. We also sequenced the quinolone resistance-determining regions (QRDRs) in type II topoisomerases and analyzed the relationship between minimum inhibitory concentration (MIC) and six specific mutations (Ser81 or Glu85 in GyrA, Asp435 in GyrB, Ser79 or Asp83 in ParC, and Asp435 in ParE). Eighteen of the isolated strains (2.7%) showed intermediate susceptibility and resistance to levofloxacin (MIC > 4µg/ml), with no significant difference among years. However, garenoxacin and sitafloxacin showed excellent activity against these strains. Of the 18 strains, 17 (94.4%) showed mutation in QRDRs, while in 12 out of 14 levofloxacin-resistant strains (85.7%) two or more mutations were identified. A single QRDR mutation was found in 3 of 60 levofloxacin-susceptible strains (5%), all of which had an MIC of 2µg/ml. We therefore found a high isolation frequency of levofloxacin-resistant S. pneumonia in Japan over a 2-year period. Furthermore, QRDR mutations were present in 5% of susceptible strains; these were thought to be in the early stages of resistance. In the future, the increasing use of levofloxacin might result in more strain resistance. We therefore suggest that strong quinolones such as garenoxacin and sitafloxacin could be proactively administered to high-risk patients. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.26.181 | |||||
出版者 | ||||||
出版者 | Showa University Society | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |