WEKO3
アイテム
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A profile of pro-inflammatory cytokine expression in human Delta-1-induced monocyte-derived Langerhans cell-like dendritic cells after stimulation with Toll-like receptor ligands
https://showa.repo.nii.ac.jp/records/3839
https://showa.repo.nii.ac.jp/records/3839c6d07d87-bd25-42c8-b7ea-ea9194d14d12
名前 / ファイル | ライセンス | アクション |
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S33_67.pdf (386.0 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2021-09-22 | |||||
タイトル | ||||||
タイトル | A profile of pro-inflammatory cytokine expression in human Delta-1-induced monocyte-derived Langerhans cell-like dendritic cells after stimulation with Toll-like receptor ligands | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
TAKAHASHI, Rei
× TAKAHASHI, Rei× IWAMOTO, Sanju× TANIOKA, Toshihiro× MAEDA, Kohei |
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書誌情報 |
The Showa University journal of medical sciences 巻 33, 号 3, p. 67-73, 発行日 2021-09 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Monocyte-derived Langerhans cell-like dendritic cells (Mo-LCs) are involved in epidermal disorders such as psoriasis in murine models. However, the roles of Mo-LCs in the pathogenesis of psoriasis in humans remain unclear. Also, the contribution of notch ligand delta-like 1 (DLL-1), expressed on keratinocytes, to Mo-LC functions requires clarification. Here, we established a new method of stimulating Mo-LCs derived from CD14+ monocytes with immobilized human DLL-1 to generate induced Mo-LCs (DI(+)Mo-LCs). The DI(+)Mo-LCs were compared to the dendritic cells derived from monocytes (Mo-DCs) cultured with interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF), and M1 macrophages (Mφ) derived from monocytes cultured with GM-CSF. The DI(+)Mo-LCs were found to produce significant amounts of IL15, IL23A, and interferon-β (IFNB1) in response to the Toll-like receptor (TLR)3 agonist Polyinosinic-polycytidylic acid (Poly(I:C)) or TLR4 agonist lipopolysaccharide (LPS) despite their low expression of tumor necrosis factor (TNF). In conclusion, we have established a new method to generate DI(+)Mo-LCs. We have also discovered that DI(+)Mo-LCs have a unique capacity for producing IL15 and IL23A, which are related to the pathogenesis of psoriasis. Our data contribute to a better understanding of the roles of Mo-LCs in epidermal defense and pathogenesis. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.33.67 | |||||
出版者 | ||||||
出版者 | Showa University Society | |||||
ISSN | ||||||
収録物識別子 | 2185-0968 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |