WEKO3
アイテム
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Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β - catenin Signaling Pathway in Triple-negative Breast Cancer
https://showa.repo.nii.ac.jp/records/2953
https://showa.repo.nii.ac.jp/records/2953bb0cdca5-0497-405a-aa2d-c04cb471a561
名前 / ファイル | ライセンス | アクション |
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S30_359.pdf (1.3 MB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-08-05 | |||||
タイトル | ||||||
タイトル | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β - catenin Signaling Pathway in Triple-negative Breast Cancer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
OKAZAKI, Keinosuke
× OKAZAKI, Keinosuke× SASAKI, Akiko× TSUNODA, Yuko× FURUYA, Kanji× TSUJI, Mayumi× UDAKA, Yuko× OYAMADA, Hideto× HOSONUMA, Masahiro× SHIRAKO, Haruna× YASUMOTO, Taro× KIUCHI, Yuji× SASAKI, Tadanori |
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書誌情報 |
The Showa University journal of medical sciences 巻 30, 号 3, p. 359-370, 発行日 2018-09 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Triple-negative breast cancer (TNBC) accounts for 10-15% of all breast cancer cases and shows a poor prognosis with 30% distant metastasis. With few specific target molecules and ineffective hormonal and anti-HER2 treatment, an alternative therapeutic method for TNBC is urgently required. Recently, a non-taxane inhibitor of microtubule dynamics called eribulin was developed for breast cancer therapy. Eribulin induces irreversible mitotic mass formation in cancer cells during the G2-M phase, initiating apoptosis; however, the mechanism underlying this eribulin activity remains unclear. We reported previously that exposing non-basal-like (NBL) TNBC cells to eribulin increases miR-195 expression, which in turn decreases the expression of targeted Wnt3a. The present study sought to further clarify the mechanism of this antitumor effect by exploring how eribulin affects Wnt/β - catenin signaling based on miRNA expression changes in TNBC. In an NBL type of human breast cancer cell line (MDA-MB-231 cells), we compared the expression levels of Wnt/β catenin signaling pathway proteins in cells exposed to an miR-195 mimic (cells transfected with miR-195 and in which Wnt3a expression was suppressed) and in cells exposed to eribulin. Expression levels of Wnt3a, β -catenin, and GSK-3β were measured by ELISA and observed by fluorescence immunostaining. Wnt3a and β -catenin expression was significantly lower and GSK-3β expression was significantly higher in the cells exposed to eribulin and transfected with miR-195 mimic than in the untreated controls, suggesting that eribulin inactivates the Wnt/β -catenin signaling pathway. Therefore, a novel antitumor mechanism of eribulin was determined, whereby eribulin induces high expression of miR-195 to inactivate the Wnt/β -catenin signaling pathway in NBL-type TNBC. | |||||
DOI | ||||||
関連識別子 | 10.15369/sujms.30.359 | |||||
出版者 | ||||||
出版者 | Showa University Society | |||||
ISSN | ||||||
収録物識別子 | 0915-6380 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |