WEKO3
アイテム
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Gene Expression of Cancer Stem Cell in Oral Squamous Cell Carcinoma
https://showa.repo.nii.ac.jp/records/1043
https://showa.repo.nii.ac.jp/records/1043eae68adb-6e02-49bd-89c0-b937c92c21f0
名前 / ファイル | ライセンス | アクション |
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D32_81.pdf (3.6 MB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-03-31 | |||||
タイトル | ||||||
タイトル | Gene Expression of Cancer Stem Cell in Oral Squamous Cell Carcinoma | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
ISOBE, Tomohide
× ISOBE, Tomohide× Yamamoto, Gou× IRIE, Tarou× TACHIKAWA, Tetuhiko× MISHIMA, Kenji |
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書誌情報 |
Dental medicine research 巻 32, 号 2, p. 81-89, 発行日 2012-07 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Oral squamous cell carcinoma (OSCC), like many solid tumors, contains a heterogeneous population of cancer cells. Recent data suggest that a rare subpopulation of cancer cells, known as cancer stem cells (CSCs), is capable of initiating, maintaining, and expanding the growth of tumors. Identification and characterization of CSCs from OSCC would facilitate the monitoring, therapy, or prevention of this cancer. CD133 is considered a marker molecule for CSCs; however, its role in OSCC is yet to be determined. In this study, we isolated CD133-positive cells from OSCC cell lines using a magnetic-activated cell sorter (MACS). The differential expression of genes between OSCC/CD133-positive cells and parental cells was determined by polymerase chain reaction (PCR). Up-regulated genes (CD133-positive vs. parental cells) included ALDH 1, Keratin15 (Krt15), SOX2, and WNT 1, while the down-regulated genes included Fgfr1 and Pparg. Additionally, immunohistochemical analysis revealed that expression of Krt15 and SOX2 was localized to cancer cells of OSCC specimens. Their elevated expression levels were detected in poorly differentiated and chemoresistant OSCC. Our results possibly demonstrate that CD133-positive cells, when compared with parental cells, are a more concentrated population of CSCs in OSCC. | |||||
DOI | ||||||
関連識別子 | 10.7881/dentalmedres.32.81 | |||||
出版者 | ||||||
出版者 | 昭和大学・昭和歯学会 | |||||
ISSN | ||||||
収録物識別子 | 1882-0719 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |